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Journal / Immunology and Infection / Assessing the Expression of Major…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Assessing the Expression of Major Histocompatibility Complex Class I on Primary Murine Hippocampal Neurons by Flow Cytometry
 

Assessing the Expression of Major Histocompatibility Complex Class I on Primary Murine Hippocampal Neurons by Flow Cytometry

Article DOI: 10.3791/61436-v • 08:07 min • May 19th, 2020
May 19th, 2020 •
1, 1
1Department of Biological Sciences, University of North Carolina at Charlotte

Riepilogo
Automatic Translation

This protocol describes a method for culturing primary hippocampal neurons from embryonic mouse brain. The expression of major histocompatibility complex class I on the extracellular surface of cultured neurons is then assessed by flow cytometric analysis.

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Keywords: Flow Cytometry MHC Class I Primary Neurons Hippocampus Embryonic Brain Dissection Papain Dissociation Hibernate-E Medium Trituration
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