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Precise Brain Mapping to Perform Repetitive In Vivo Imaging of Neuro-Immune Dynamics in Mice
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Capitoli
- 00:04Introduction
- 00:51Mouse Preparation for Cranial Window Implantation
- 02:20Cranial Window Implantation Surgery
- 03:57Two-photon Brain Mapping and Imaging
- 05:24Results: Visualizing Microglial Dynamics In Vivo
- 07:26Conclusion
This protocol describes a chronic cranial window implantation technique that can be used for longitudinal imaging of neuro-glio-vascular structures, interactions, and function in both healthy and diseased conditions. It serves as a complementary alternative to the transcranial imaging approach that, while often preferred, possesses some critical limitations.
