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An Ex vivo Assay to Study Candida albicans Hyphal Morphogenesis in the Gastrointestinal Tract
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
An Ex vivo Assay to Study Candida albicans Hyphal Morphogenesis in the Gastrointestinal Tract

An Ex vivo Assay to Study Candida albicans Hyphal Morphogenesis in the Gastrointestinal Tract

DOI:
10.3791/61488-v

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07:42 min

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July 01, 2020

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, ,
1College of Veterinary Medicine,Midwestern University, 2Masters in Biomedical Science Program, College of Graduate Studies,Midwestern University, 3Department of Pathology and Population Medicine, College of Veterinary Medicine,Midwestern University

Capitoli

  • 00:04Introduction
  • 00:35Mouse Dissection
  • 02:33Ex Vivo Prep and Exogenous Addition of Metabolites to the Gut Homogenate Extracts for Hyphal Morphogenesis Assay
  • 04:21C. albicans Morphogenesis Assay
  • 05:50Results: Effects of Antibiotic and Gut Metabolites on C. albicans Hyphal Morphogenesis
  • 06:58Conclusion

Summary

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The ex vivo assay described in this study using gut homogenate extracts and immunofluorescence staining represents a novel method to examine the hyphal morphogenesis of Candida albicans in the GI tract. This method can be utilized to investigate the environmental signals regulating morphogenetic transition in the gut.

Tags

Keywords: Ex Vivo AssayCandida AlbicansHyphal MorphogenesisGastrointestinal TractGut MetabolitesEnteric PathogensDissectionGI Tract ExtractionGut Content CollectionC. Albicans CulturePBS ResuspensionGut Content HomogenizationMicroscopy Analysis

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