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En Ex vivo Assay at studere Candida albicans Hyphal Morphogenese i mave-tarmkanalen
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
An Ex vivo Assay to Study Candida albicans Hyphal Morphogenesis in the Gastrointestinal Tract

En Ex vivo Assay at studere Candida albicans Hyphal Morphogenese i mave-tarmkanalen

DOI:
10.3791/61488-v

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07:42 min

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July 01, 2020

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, ,
1College of Veterinary Medicine,Midwestern University, 2Masters in Biomedical Science Program, College of Graduate Studies,Midwestern University, 3Department of Pathology and Population Medicine, College of Veterinary Medicine,Midwestern University

Capitoli

  • 00:04Introduction
  • 00:35Mouse Dissection
  • 02:33Ex Vivo Prep and Exogenous Addition of Metabolites to the Gut Homogenate Extracts for Hyphal Morphogenesis Assay
  • 04:21C. albicans Morphogenesis Assay
  • 05:50Results: Effects of Antibiotic and Gut Metabolites on C. albicans Hyphal Morphogenesis
  • 06:58Conclusion

Summary

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Ex vivo-analysen, der er beskrevet i denne undersøgelse ved hjælp af guthomogenatekstrakter og immunofluorescensfarvning, repræsenterer en ny metode til at undersøge hyphal morfogensen af Candida albicans i mave-tarmkanalen. Denne metode kan anvendes til at undersøge de miljømæssige signaler, der regulerer morfogenetiske overgang i tarmen.

Tags

Keywords: Ex Vivo AssayCandida AlbicansHyphal MorphogenesisGastrointestinal TractGut MetabolitesEnteric PathogensDissectionGI Tract ExtractionGut Content CollectionC. Albicans CulturePBS ResuspensionGut Content HomogenizationMicroscopy Analysis

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