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1Chronobiology of Reproduction Research Lab, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza,Universidad Nacional Autónoma de México, 2Developmental Biology Lab, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza,Universidad Nacional Autónoma de México, 3Neuroendocrinology Lab, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza,Universidad Nacional Autónoma de México, 4Facultad de Ciencias,Universidad Nacional Autónoma de México, 5Department of Basic Research, Instituto Nacional de Geriatria,Ministry of Health
Capitoli
- 00:04Introduction
- 00:47Construction of Bilateral Cannulas, Obturators, and Caps
- 02:49Construction of Microinjectors
- 04:10Animal Maintenance and Vaginal Smears
- 05:31Microinjection of TTX or Vehicle Solutions into Freely Moving Rats
- 07:27Tissue Processing
- 08:44Results: Percentage of Ovulating Animals and Median of the Number of Ova Shed after Microinjection with Artificial Cerebrospinal Fluid (ACSF) or Tetrodotoxin (TTX)
- 10:08Conclusion
