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静脉畸形的患者衍生异种移植模型
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
A Patient-Derived Xenograft Model for Venous Malformation
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静脉畸形的患者衍生异种移植模型

DOI:
10.3791/61501-v

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06:51 min

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June 15, 2020

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, ,
1Division of Experimental Hematology and Cancer Biology,Cincinnati Children’s Hospital Medical Center, 2Department of Pediatrics,University of Cincinnati College of Medicine

Capitoli

  • 00:04Introduction
  • 00:42VM Patient-derived Xenograft Protocol
  • 03:11Tissue Collection and Processing
  • 03:45Analysis of Human-derived Vascular Channels
  • 05:13Results: VM Lesion Plug
  • 06:10Conclusion

Summary

Traduzione automatica

Traduzione automatica

我们提出了一个详细的协议，以生成静脉畸形的杂音异种模型。该模型基于患者衍生的内皮细胞的皮下注射，这些内皮细胞含有超激活的 TIE2 和/或 PIK3CA 基因突变。异种移植病变密切回顾VM患者组织组织组织病理学特征。

Tags

Patient-derived XenograftVenous MalformationPreclinical Therapeutic TestingEndothelial CellsVascular AnomaliesTrypsinizationCell SuspensionCentrifugationBMEMAnesthesiaInjectionCaliper MeasurementFormalin Fixation

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