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1Institute of Integrated Clinical Practice, Faculty of Medicine,University of Coimbra, Coimbra, Portugal, 2Coimbra Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO),University of Coimbra, Coimbra, Portugal, 3Center for Innovative Biomedicine and Biotechnology (CIBB),University of Coimbra, Coimbra, Portugal, 4Clinical Academic Center of Coimbra, CACC, Portugal, 5Institute of Biophysics, Faculty of Medicine,University of Coimbra, Coimbra, Portugal, 6Laboratory of Oncobiology and Hematology, Faculty of Medicine,University of Coimbra, Coimbra, Portugal, 7Institute of Endodontics, Faculty of Medicine,University of Coimbra, Coimbra, Portugal, 8Institute of Experimental Pathology, Faculty of Medicine,University of Coimbra, Coimbra, Portugal
Capitoli
- 00:05Introduction
- 01:12Pellet Preparation and Soluble Biomaterial Extracts Acquisition
- 02:11Morphology Evaluation
- 03:28Cell Function Assessment
- 05:54Mineralized Formation Assessment
- 06:50Results: Cell Viability, Morphology, and Protein Expression in Response to Biomaterial Exposure
- 08:30Conclusion
Questa metodologia mira a valutare la citotossicità dei biomateriali attraverso la preparazione di estratti solubili, utilizzando saggi di vitalità e analisi fenotipiche, tra cui citometria a flusso, RT-PCR, immunocitochimica e altre tecniche di biologia cellulare e molecolare.
