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Obtaining Primary Osteocytes Through Murine Calvarial Fractionation of GFP-Expressing Osteocytes
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Biologia
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JoVE Journal Biologia
Obtaining Primary Osteocytes Through Murine Calvarial Fractionation of GFP-Expressing Osteocytes

Obtaining Primary Osteocytes Through Murine Calvarial Fractionation of GFP-Expressing Osteocytes

DOI:
10.3791/61513-v

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07:22 min

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June 02, 2020

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, , , , , , , , , ,
1Division of Orthodontics and Dentofacial Orthopedics, Department of Translational Medicine,Tohoku University Graduate School of Dentistry

Capitoli

  • 00:05Introduction
  • 00:44Newborn Mouse Calvaria Dissection
  • 01:38Newborn Mouse Calvaria Fractionation
  • 03:47Osteocyte Preparation for Fluorescence Activated Cell Sorting
  • 05:13Results: Representative Osteocyte Characterization
  • 06:44Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol describes the dissection of neonatal dmp1-topaz mouse calvaria and isolation of osteocytes expressing the green fluorescent protein through cell digestion and fractionation, in addition to osteocyte preparation for fluorescence activated cell sorting (FACS).

Tags

Primary OsteocytesCalvarial FractionationGFP-expressing OsteocytesOsteocyte IsolationCell CultureCollagenaseEDTACell FractionationCell Purification

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