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Whole Animal Imaging of Drosophila melanogaster using Microcomputed Tomography
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Whole Animal Imaging of Drosophila melanogaster using Microcomputed Tomography

Whole Animal Imaging of Drosophila melanogaster using Microcomputed Tomography

DOI:
10.3791/61515-v

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10:36 min

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September 02, 2020

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1Department of Molecular Biology,University of Wyoming

Capitoli

  • 00:05Introduction
  • 00:44Sample Selection, Cuticle Preparation, Fixation, and Staining
  • 02:52Sample Mounting
  • 03:46Scanning
  • 05:51Reconstruction and Image Analysis
  • 08:07Results: Representative Drosophila melanogaster Imaging
  • 09:56Conclusion

Summary

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A protocol is presented that allows for the visualization of intact Drosophila melanogaster at any stage of development using microcomputed tomography.

Tags

Keywords: Drosophila MelanogasterMicrocomputed TomographyWhole Animal ImagingNon-destructive ImagingDevelopmental DefectsDisease ModelingSample PreparationBouin SolutionPBSStainingCritical Point DryingSample Mounting

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