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牛磺胆酸钠诱导C57BL / 6小鼠严重急性胰腺炎

JoVE Journal
Medicina

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JoVE Journal Medicina

Sodium Taurocholate Induced Severe Acute Pancreatitis in C57BL/6 Mice

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牛磺胆酸钠诱导C57BL / 6小鼠严重急性胰腺炎

DOI:

10.3791/61547-v

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06:35 min

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June 28, 2021

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Mariana B. Serra*¹, Márcia K. Koike*¹, Denise F. Barbeiro*¹, Marcel C. C. Machado*¹, Heraldo P. de Souza*¹

¹Discipline of Clinical Emergencies, Department of Clinical Medicine, HCFMUSP / LIM 51,University of São Paulo Medical School

Capitoli

00:04Introduction
01:07Laparotomy and Exposing the Pancreas
02:17Severe Acute Pancreatitis Induction
03:30Methods for Analysis
05:13Results: Analysis of Histological and Serological Changes after Induction of Acute Pancreatitis
06:00Conclusion

Summary

Traduzione automatica

严重急性胰腺炎的动物模型能够在初始阶段研究病理生理变化，促进观察炎症事件的演变。在这里，我们提供了通过将牛磺草酸钠逆行输注到麻醉的C57BL / 6小鼠的胰管中来诱导严重急性胆汁性胰腺炎的方案。

Tags

Keywords: Acute Pancreatitis C57BL/6 Mice Sodium Taurocholate Biliary Pancreatitis Inflammatory Response Pharmacological Intervention Laparotomy Common Bile Duct Duodenum Infusion Animal Model

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