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Planarian Scrunching as a Quantitative Behavioral Readout for Noxious Stimuli Sensing
JoVE Journal
Biologia
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JoVE Journal Biologia
Planarian Scrunching as a Quantitative Behavioral Readout for Noxious Stimuli Sensing
DOI:

10:15 min

July 30, 2020

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Capitoli

  • 00:04Introduction
  • 00:44Quantitative Planarian Behavior Assays
  • 02:34Data Analysis
  • 05:59Scrunching Induction
  • 07:41Results: Species Specific Responses to Near UV Light and Cinnamaldehyde Exposure
  • 09:38Conclusion

Summary

Traduzione automatica

Freshwater planarians exhibit three gaits (gliding, peristalsis, and scrunching) that are distinguishable by quantitative behavioral analysis. We describe a method to induce scrunching using various noxious stimuli, quantification thereof, and distinction from peristalsis and gliding. Using gene knockdown, we demonstrate the specificity of scrunching as a quantitative phenotypic readout.

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