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An In Vitro Hemodynamic Loop Model to Investigate the Hemocytocompatibility and Host Cell Activation of Vascular Medical Devices
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JoVE Journal Medicina
An In Vitro Hemodynamic Loop Model to Investigate the Hemocytocompatibility and Host Cell Activation of Vascular Medical Devices

An In Vitro Hemodynamic Loop Model to Investigate the Hemocytocompatibility and Host Cell Activation of Vascular Medical Devices

DOI:
10.3791/61570-v

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08:44 min

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August 21, 2020

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, , , , , , , , ,
1Department of Cardiothoracic Surgery,Otto-von-Guericke-University, 2Department of Mechanical Engineering, Institute for Materials and Joining Technology,Otto-von-Guericke-University, 3Institute of Clinical Chemistry and Pathobiochemistry,Otto-von-Guericke-University, 4Division of Cardiology and Angiology, Department of Internal Medicine,Otto-von-Guericke-University

Capitoli

  • 00:04Introduction
  • 00:43In Vitro Hemodynamic Loop Assembly and Blood Collection
  • 02:59Blood Sample Processing and Scanning Electron Microscopy (SEM) and Microcomputed Tomography (CT) Imaging Preparation
  • 04:16Flow Cytometry
  • 06:12Results: Representative Hemocytocompatibility and Host Cell Activation Analyses
  • 08:09Conclusion

Summary

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Presented here is a protocol for a standardized in vitro hemodynamic loop model. This model allows to test the hemocompatibility of perfusion tubes or vascular stents to be in accordance with ISO (International Organization for Standardization) standard 10993-4.

Tags

In Vitro Hemodynamic Loop ModelHemocytocompatibilityHost Cell ActivationVascular Medical DevicesPerfusion TubingsStentsBlood CompatibilityTube CutterTension Band ConnectorStent Loop AssemblyLoop CradleRotation UnitHeparinBlood CollectionHomogenous SolutionBlood FillingLoop Rotation

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