Rivista
/
Biologia dello sviluppo
/
牛早期蚁卵泡卵母细胞的体外培养策略
JoVE Journal
Biologia dello sviluppo
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia dello sviluppo
In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle
Please note that all translations are automatically generated. Click here for the English version.

牛早期蚁卵泡卵母细胞的体外培养策略

DOI:
10.3791/61625-v

•

09:30 min

•

July 08, 2020

•

, , ,
1Reproductive and Developmental Biology Laboratory, Department of Health, Animal Science and Food Safety,University of Milan

Capitoli

  • 00:04Introduction
  • 00:50Media Preparation
  • 01:44Ovary Collection and Processing
  • 03:03Selection and Isolation of the Follicles and Retrieval of the COCs
  • 04:31Long in Vitro Culture of the Oocytes (long IVCO
  • 07:15Results: Meiotic Progression of Oocytes after long IVCO
  • 08:52Conclusion

Summary

Traduzione automatica

Traduzione automatica

我们描述了在发育的早期阶段从卵巢卵泡分离生长的卵母细胞的过程，以及建立体外培养系统，该系统可以支持生长和分化，一次，一次，一次，一个发育阶段。

Tags

In Vitro CultureOocytesEarly Antral FolliclesCattleGenetic SalvageThreatened SpeciesLocal BreedsOvary SlicingFollicle SelectionCulture MediumCilostamideLong IVCO Medium96-well PlateIncubation Conditions

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

了解早期器官使用简体<em>原位</em>杂交协议<em>爪</em

评定神经连接的形成在共培养体系的光遗传学方法

干细胞样<em>爪</em>胚胎外植体，研究早期神经发育特点<em>体外</em>和<em>在体内</em

基于核仁染色质组织分离鼠标窦卵母细胞与表征

人多能干细胞对原始和最终造血祖细胞定向分化的研究

斑马鱼早期胚胎和肿瘤细胞电活动的可视化研究

无支架三维胰岛素表达 Pancreatoids 的实验研究体外培养的小鼠胰祖细胞

人诱导多能干细胞体外生成体细胞衍生物的研究

人类羊膜不同解剖区域的皮下细胞体外培养

从人类诱导多能干细胞诱导的体外神经肌肉结点

Read Article