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1Laboratory of Biomedical Embryology, Department of Health, Animal Science and Food Safety and Center for Stem Cell Research,Università degli Studi di Milano, 2Department of Veterinary Medicine,University of Sassari, 3Department of Agricultural and Environmental Sciences – Production, Landscape, Agroenergy and Center for Stem Cell Research,Università degli Studi di Milano
Capitoli
- 00:04Introduction
- 01:11Fibroblast Exposure to 5-aza-CR
- 02:28Fibroblast Encapsulation in PTFE Micro-Bioreactors
- 04:05Culture in PTFE Micro-Bioreactors of Epigenetically Erased Cells
- 05:00Results: Epigenetically Erased Cells Encapsulated in PTFE Micro-Bioreactors Show High Level and Long-term Maintenance of Pluripotency-Related Gene Expression
- 07:33Conclusion
Vi præsenterer her en metode, der kombinerer brugen af kemisk epigenetisk sletning med mekanosensing-relaterede signaler til effektivt at generere pattedyrs pluripotente celler uden behov for gentransfektion eller retrovirale vektorer. Denne strategi er derfor lovende for translationel medicin og repræsenterer et bemærkelsesværdigt fremskridt inden for stamcelleorganoidteknologi.
