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Journal / Environment / Two-Dimensional Visualization and Quantification…
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Environment

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JoVE Journal Environment
Two-Dimensional Visualization and Quantification of Labile, Inorganic Plant Nutrients and Contaminants in Soil
 

Two-Dimensional Visualization and Quantification of Labile, Inorganic Plant Nutrients and Contaminants in Soil

Article DOI: 10.3791/61661-v • 12:03 min • September 1st, 2020
September 1st, 2020 •
1, 2, 1, 1,3
1Department General, Analytical and Physical Chemistry, Chair of General and Analytical Chemistry, Montanuniversität Leoben, 2Department of Forest and Soil Sciences, Institute of Soil Research, Rhizosphere Ecology and Biogeochemistry Group, University of Natural Resources and Life Sciences, Vienna, 3Department of Crop Sciences, Institute of Agronomy, University of Natural Resources and Life Sciences, Vienna

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This protocol presents a workflow for sub-mm 2D visualization of multiple labile inorganic nutrient and contaminant solute species using diffusive gradients in thin films (DGT) combined with mass spectrometry imaging. Solute sampling and high-resolution chemical analysis are described in detail for quantitative mapping of solutes in the rhizosphere of terrestrial plants.

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Keywords: Two-dimensional Visualization Labile Inorganic Plant Nutrients Contaminants Soil DGT Mass Spectrometry Imaging Nutrient And Contaminant Uptake Rhizotron Polyurethane Gel PTFE Foil
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