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心肌梗塞穆林模型中的 3D 生物打印贴片移植
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
Transplantation of a 3D Bioprinted Patch in a Murine Model of Myocardial Infarction
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心肌梗塞穆林模型中的 3D 生物打印贴片移植

DOI:
10.3791/61675-v

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07:47 min

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September 26, 2020

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,
1The University of Sydney, 2University of Technology Sydney (UTS), 3The Royal North Shore Hospital, 4University Hospital of Wales

Capitoli

  • 00:00Introduction
  • 00:42Surgical Procedure
  • 01:18Preparing the Surgical Field
  • 01:31Left Lateral Thoracotomy
  • 02:00Left Anterior Descending (LAD) Permanent Coronary Artery Ligation
  • 02:583D Bioprinting and Culturing Patches (7-14 days before transplantation)
  • 04:19Transplantation of the Bioprinted Patch onto the Epicardium
  • 05:39Mouse Recovery
  • 06:29Representative Results
  • 06:45Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议旨在移植一个3D生物打印补丁到梗死小鼠的史诗建模心力衰竭。它包括有关麻醉、手术胸腔开口、左前降（LAD）冠状动脉的永久结扎以及将生物打印的贴片应用到心脏梗塞区域的详细信息。

Tags

Keywords: 3D BioprintingStem CellHeart PatchMyocardial InfarctionCardiac CellsHydrogelLAD LigationMouse ModelTransplantation

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