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眼部微血管血管生成前维沃胆球状芽分析
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Biologia
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JoVE Journal Biologia
An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis
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眼部微血管血管生成前维沃胆球状芽分析

DOI:
10.3791/61677-v

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06:10 min

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August 06, 2020

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, , , , ,
1Department of Ophthalmology,Boston Children’s Hospital, Harvard Medical School, 2Department of Clinical and Metabolic Genetics,Hospital for Sick Children, University of Toronto, 3Manton Center for Orphan Disease,Harvard Medical School, Boston Children’s Hospital

Capitoli

  • 00:04Introduction
  • 00:47Preparation and Experimental Steps
  • 03:26SWIFT-Choroid Computerized Quantification Method
  • 04:25Results: Free Fatty Acid Receptor (FFAR) 4 Suppression Exacerbate Choroidal Neovascularization
  • 05:38Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议提供胆瘤发芽测定，微血管增殖的外体模型。这种测定可用于评估增殖胆小血管所涉及的途径，并评估使用野生型和转基因小鼠组织的药物治疗。

Tags

Keywords: Ex VivoChoroidAngiogenesisMicrovascularAge-related Macular Degeneration (AMD)Choroidal NeovascularizationBME (basal Membrane Extract)Classic MediumMicroscopy

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