È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
1GCC Center for Advanced Microscopy and Image Informatics, 2Department of Molecular and Cellular Biology,Baylor College of Medicine, 3Center for Translational Cancer Research, Institute of Biosciences and Technology,Texas A&M University, 4Department of Pharmacology and Chemical Biology,Baylor College of Medicine
Capitoli
- 00:04Introduction
- 00:34RNA Fluorescence In Situ Hybridization (FISH) Fixation
- 01:14RNA FISH Hybridization
- 02:16Imaging Preparation
- 03:08High Resolution Microscopy and Image Processing
- 04:05Image Analysis
- 04:45Results: Representative Image Segmentation and E2-Induced GREB1 Quantification
- 05:56Conclusion
Enmolekyl RNA fluorescens in situ hybridisering (smFISH) är en metod för att exakt kvantifiera nivåer och lokalisering av specifika RNAs på encellsnivå. Här rapporterar vi våra validerade labbprotokoll för våtbänksbehandling, bildbehandling och bildanalys för encells kvantifiering av specifika RNAs.
