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Optimization of Crystal Growth for Neutron Macromolecular Crystallography
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Biologia
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JoVE Journal Biologia
Optimization of Crystal Growth for Neutron Macromolecular Crystallography

Optimization of Crystal Growth for Neutron Macromolecular Crystallography

DOI:
10.3791/61685-v

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12:29 min

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March 13, 2021

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, ,
1CEA, CNRS, IBS,Université Grenoble Alpes, 2ELVESYS SAS

Capitoli

  • 00:00Introduction
  • 01:49Protocol: Instrument/ Flow-cell
  • 03:59Protocol: Software
  • 07:33Risultati
  • 11:23Conclusion

Summary

Traduzione automatica

Traduzione automatica

Structural studies of biomacromolecules by crystallography require high-quality crystals. Here we demonstrate a protocol that can be used by OptiCrys (a fully automated instrument developed in our lab) and/or microdialysis buttons for growing large high-quality crystals based on knowledge of the crystallization phase diagram.

Tags

Keywords: Crystal GrowthNeutron Macromolecular CrystallographyDialysis MethodTemperature-controlled Dialysis CrystallizationMicro Dialysis ButtonsLysozymeNucleationMetastable ZoneOptiCrysPhase DiagramProtein Crystallization

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