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Genetics

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JoVE Journal Genetics
在Vivo CRISPR/Cas9筛查中同时评估小鼠皮肤和口腔中的基因功能
 
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在Vivo CRISPR/Cas9筛查中同时评估小鼠皮肤和口腔中的基因功能

Article DOI: 10.3791/61693-v • 07:52 min • November 2nd, 2020
November 2nd, 2020 •
1, 1,2, 1,2, 3, 1,2
1Centre for Molecular and Systems Biology, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 2Department of Molecular Genetics, University of Toronto, 3Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University

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在这里，我们描述了一种快速和直接的体内CRISPR/Cas9筛查方法，使用超声波引导子宫胚胎扁平注射，同时评估几个基因在皮肤和免疫能力小鼠口腔的功能。

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本月在JoVE，第165期，在体内CRISPR，超声引导微注射，长尾，CRISPR库，小鼠癌症模型，在体内屏幕快速
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