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样品室光片荧光显微镜中多个昆虫胚胎的同步实时成像
JoVE Journal
Biologia
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JoVE Journal Biologia
Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes
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样品室光片荧光显微镜中多个昆虫胚胎的同步实时成像

DOI:
10.3791/61713-v

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08:29 min

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September 09, 2020

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, ,
1Physical Biology/Physikalische Biologie (IZN, FB15), Buchmann Institute for Molecular Life Sciences (BMLS), Cluster of Excellence Frankfurt, Macromolecular Complexes (CEF – MC),Goethe-Universität

Capitoli

  • 00:04Introduction
  • 00:39Light Sheet Fluorescence Microscope Calibration
  • 02:37Sodium Hypochlorite-Based Dechorionation
  • 03:48Embryo Mounting
  • 04:51Comparative Live Imaging
  • 06:02Imaged Embryo Retrieval and Cultivation
  • 06:52Results: Representative Drosophila and Tribolium Fluorescence Live Imaging
  • 07:56Conclusion

Summary

Traduzione automatica

Traduzione automatica

光片荧光显微镜是发育生物学中最有价值的工具。比较研究中的一个主要问题是环境差异。我们的协议描述了多个标本同时进行实时成像的实验框架，因此，主动解决这个问题。

Tags

Light Sheet Fluorescence MicroscopySample ChamberCobweb HolderFluorescent MicrospheresEmbryo DechorionationSimultaneous Live ImagingHigh Content ImagingCalibrationAxial Resolution

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