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Preparation of Virus-Enriched Inoculum for Oral Infection of Honey Bees (Apis mellifera)
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JoVE Journal Biologia
Preparation of Virus-Enriched Inoculum for Oral Infection of Honey Bees (Apis mellifera)

Preparation of Virus-Enriched Inoculum for Oral Infection of Honey Bees (Apis mellifera)

DOI:
10.3791/61725-v

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06:41 min

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August 26, 2020

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, ,
1Department of Entomology,University of Illinois, 2Department of Microbiology,Center for Scientific Research and Higher Education of Ensenada (CICESE)

Capitoli

  • 00:04Introduction
  • 00:41Larval Self-Removal
  • 02:08Pupal Virus Injection
  • 03:19Viral Feeding Bioassay
  • 04:58Results: Representative Virus Load and Survival Curve Analyses
  • 05:55Conclusion

Summary

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Here we describe two protocols: first to propagate, extract, purify, and quantify large quantities of honey bee non-enveloped virus particles, including a method for removing honey bee pupae and second to test the effects of viral infection using a highly repeatable, high-throughput cage bioassay.

Tags

Virus-enriched InoculumOral InfectionHoney BeesApis MelliferaVirus ParticlesBioassaysVirus TreatmentBee PupaeVirus ProductionPollinator SystemsVirus InfectivityMass Bee ExtractionHoney Bee QueenLarval Self-removalLangstroth FrameIncubatorPBSVirus InjectionManual Multipipette

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