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Visualisering af Calcitonin Gene-Related Peptid Immunoreactive Innervation af Rat Cranial Dura Mater med immunfluorescens og neural sporing
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing
DOI:

07:28 min

January 06, 2021

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Chapters

  • 00:04Introduction
  • 00:45Innervation of Rat Cranial Dura Mater
  • 02:34Retrograde Tracing Study with Fluorogold
  • 05:20Results: Neurovascular Structure of the Cranial Dura Mater and Retrograde-labeled Neurons in the TG and DRGs
  • 07:02Conclusion

Summary

Automatic Translation

Her præsenterer vi en protokol til visualisering af rumlig korrelation af calcitonin genrelateret peptid (CGRP)-immunoreaktive nervefibre og blodkar i kraniedura mater ved hjælp af immunfluorescens og fluorescerende histokemi med henholdsvis CGRP og falloidin. Derudover var oprindelsen af disse nervefibre retrograd spores med en fluorescerende neurale sporstof.

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