Rivista
/
Ricerca sul cancro
/
Identification of EGFR and RAS Inhibitors using Caenorhabditis elegans
JoVE Journal
Ricerca sul cancro
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Ricerca sul cancro
Identification of EGFR and RAS Inhibitors using Caenorhabditis elegans

Identification of EGFR and RAS Inhibitors using Caenorhabditis elegans

DOI:
10.3791/61788-v

•

08:12 min

•

October 05, 2020

•

, , , , ,
1Department of Diagnostic and Biomedical Sciences, School of Dentistry,University of Texas Health Science Center, 2Department of Integrative Biology and Pharmacology, McGovern Medical School,University of Texas Health Science Center

Capitoli

  • 00:05Introduction
  • 00:26Preparation of a Synchronous C. elegans Culture
  • 02:51Preparation of Drug Assays
  • 04:30Observation of the Muv Phenotype in the let-60, let-23 and lin-1 Strains
  • 06:38Results: R-fendiline Alters let-60 and let-23 Function in C. elegans in a Dose-dependent Manner
  • 07:25Conclusion

Summary

Traduzione automatica

Traduzione automatica

The genetically tractable nematode Caenorhabditis elegans can be used as a simple and inexpensive model for drug discovery. Described here is a protocol to identify anticancer therapeutics that inhibit the downstream signaling of RAS and EGFR proteins.

Tags

EGFRRASCaenorhabditis ElegansDrug DiscoveryAnti-EGFRAnti-RASTherapeuticsE. Coli OP50NGM PlatesGravid Adult WormsWorm LysisEmbryosL1 LarvaeM9W

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/61788?article_type=v

Sectioning Mammary Gland Whole Mounts for Lesion Identification
check_url/it/61788?article_type=v

Identification and Characterization of Metastatic Factors by Gene Transfer into the Novel RIP-Tag; RIP-tva Murine Model
check_url/it/61788?article_type=v

Establishing Dual Resistance to EGFR-TKI and MET-TKI in Lung Adenocarcinoma Cells In Vitro with a 2-step Dose-escalation Procedure
check_url/it/61788?article_type=v

Identification of OTX1 and OTX2 As Two Possible Molecular Markers for Sinonasal Carcinomas and Olfactory Neuroblastomas
check_url/it/61788?article_type=v

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays
check_url/it/61788?article_type=v

Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models
check_url/it/61788?article_type=v

Characterization of the Effects of Migrastatic Inhibitors on 3D Tumor Spheroid Invasion by High-resolution Confocal Microscopy
check_url/it/61788?article_type=v

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
check_url/it/61788?article_type=v

Assays for Validating Histone Acetyltransferase Inhibitors
check_url/it/61788?article_type=v

Assessing Cellular Target Engagement by SHP2 (PTPN11) Phosphatase Inhibitors

Read Article