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使用卡诺哈布迪炎埃莱甘斯识别 EGFR 和 RAS 抑制剂
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JoVE Journal Ricerca sul cancro
Identification of EGFR and RAS Inhibitors using Caenorhabditis elegans
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使用卡诺哈布迪炎埃莱甘斯识别 EGFR 和 RAS 抑制剂

DOI:
10.3791/61788-v

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08:12 min

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October 05, 2020

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, , , , ,
1Department of Diagnostic and Biomedical Sciences, School of Dentistry,University of Texas Health Science Center, 2Department of Integrative Biology and Pharmacology, McGovern Medical School,University of Texas Health Science Center

Capitoli

  • 00:05Introduction
  • 00:26Preparation of a Synchronous C. elegans Culture
  • 02:51Preparation of Drug Assays
  • 04:30Observation of the Muv Phenotype in the let-60, let-23 and lin-1 Strains
  • 06:38Results: R-fendiline Alters let-60 and let-23 Function in C. elegans in a Dose-dependent Manner
  • 07:25Conclusion

Summary

Traduzione automatica

Traduzione automatica

基因可处理的线虫 卡诺哈布迪炎的叶原体 可以用作药物发现一个简单和廉价的模型。这里描述的是一个协议，以确定抗癌治疗，抑制RAS和EGFR蛋白的下游信号。

Tags

EGFRRASCaenorhabditis ElegansDrug DiscoveryAnti-EGFRAnti-RASTherapeuticsE. Coli OP50NGM PlatesGravid Adult WormsWorm LysisEmbryosL1 LarvaeM9W

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