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Immunologia e infezioni

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使用下一代测序的 T 和 B 细胞受体免疫再剧目分析

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

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使用下一代测序的 T 和 B 细胞受体免疫再剧目分析

DOI:

10.3791/61792-v

•

08:59 min

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January 12, 2021

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Lael Werner², Chen Dor⁴, Naomi Salamon, Meital Nagar⁴, Dror S. Shouval²

¹Pediatric Gastroenterology Unit, Edmond and Lily Safra Children’s Hospital,Sheba Medical Center, ²Sackler Faculty of Medicine,Tel Aviv University, ³Cancer Research Center,Sheba Medical Center, ⁴Molecular Hematology Laboratory,Sheba Medical Center

Capitoli

00:05Introduction
00:48DNA Isolation and Library Preparation
04:21Amplicon Purification and Quantification
05:57Next-generation Sequencing
06:57Results: TCRβ and IGH Immune Repertoire Data Analysis
08:27Conclusion

Summary

Traduzione automatica

当前协议描述了从血液样本和肠道活检中分离脱氧核糖核酸的方法、用于下一代测序的 TCR® 和 IGH PCR 库的生成、NGS 运行的性能和基本数据分析。

Tags

Keywords: T Cell Receptor B Cell Receptor Next-generation Sequencing Lymphocyte Diversity Biomarkers DNA Isolation Cell Lysis Proteinase K Protein Precipitation DNA Extraction

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