/
/
Transillumination-assisteret dissektion af specifikke stadier af musen Seminiferous Epitel Cycle for Downstream Immunostaining Analyser
This content is Free Access.
1Institute of Biomedicine, Integrative Physiology and Pharmacology Unit,University of Turku, Turku, Finland, 2Department of Pediatrics,Turku University Hospital, Turku, Finland, 3Centre for Reproductive Health,Hudson Institute of Medical Research, Melbourne, VIC 3168, Australia, 4Australian Regenerative Medicine Institute,Monash University, Melbourne, VIC 3800, Australia
Capitoli
- 00:04Introduction
- 00:51Testis Dissection and Preparation
- 02:10Transillumination-Assisted Microdissection
- 02:47Squash Preparation and Stage Verification
- 04:04Whole-Mount Seminiferous Tubule Immunostaining
- 07:04Results: Representative Mouse Seminiferous Epithelial Cycle Staging
- 09:16Conclusion
Denne protokol beskriver transilluminationsassisteret mikrodissection af segmenter af voksne mus seminiferous tubules, der repræsenterer specifikke stadier af seminiferous epitelcyklus, og celletyper deri, og efterfølgende immunfarvning af squashpræparater og intakte tubule segmenter.
