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Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
JoVE Journal
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Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

DOI:
10.3791/61830-v

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06:54 min

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October 27, 2020

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, , , ,
1Oncode Institute and Department of Cell Chemical Biology,Leiden University Medical Center

Capitoli

  • 00:05Introduction
  • 01:57Analysis of TGF-β-induced EMT
  • 04:17Results: TGF-β Signaling Responses
  • 06:20Conclusion

Summary

Traduzione automatica

Traduzione automatica

We describe a systematic workflow to investigate TGF-β signaling and TGF-β-induced EMT by studying the protein and gene expression involved in this signaling pathway. The methods include Western blotting, a luciferase reporter assay, qPCR, and immunofluorescence staining.

Tags

Keywords: TGF-betaEpithelial-to-mesenchymal Transition (EMT)Breast CancerNMuMG CellsE-cadherinF-actinCell MigrationCell InvasionMetastasisChemotherapy ResistanceIndirect Immunofluorescent StainingCell Density

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