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An Antegrade Perfusion Method for Cardiomyocyte Isolation from Mice
JoVE Journal
Biologia
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JoVE Journal Biologia
An Antegrade Perfusion Method for Cardiomyocyte Isolation from Mice

An Antegrade Perfusion Method for Cardiomyocyte Isolation from Mice

DOI:
10.3791/61866-v

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08:33 min

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May 19, 2021

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, , ,
1Department of Physiology,Shiga University of Medical Science

Capitoli

  • 00:04Introduciton
  • 00:48Mouse Heart Excision and Aorta Preparation
  • 02:35Antegrade Mouse Heart Perfusion
  • 04:26Individual Heart Cell Isolation
  • 06:37Results: Representative Isolated Cardiomyocyte Imaging and Analysis
  • 07:52Conclusion

Summary

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We developed a simple method for isolating high quality individual mouse heart cells by the antegrade perfusion technique. This method is Langendorff-free and useful for isolating ventricular and atrial myocytes or interstitial cells, such as cardiac fibroblasts or progenitors.

Tags

Mouse Cardiomyocyte IsolationAntegrade PerfusionLangendorff-freeHeart Cell IsolationCardiac Myocyte IsolationCIB-EGTAPerfusion PlateInfusion PumpVascular Clamp

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