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长期，无血清培养有机小鼠视网膜外植与完整的视网膜色素上皮

JoVE Journal
Neuroscienze

This content is Free Access.

JoVE Journal Neuroscienze

Long-Term, Serum-Free Cultivation of Organotypic Mouse Retina Explants with Intact Retinal Pigment Epithelium

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长期，无血清培养有机小鼠视网膜外植与完整的视网膜色素上皮

DOI:

10.3791/61868-v

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05:52 min

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November 25, 2020

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Soumaya Belhadj*¹, Arianna Tolone*¹, Gustav Christensen, Soumyaparna Das, Yiyi Chen, François Paquet-Durand

¹Institute for Ophthalmic Research,University of Tübingen

Capitoli

00:04Introduction
01:07Explant Preparation
03:46Results: Analysis of Cell Types Found in Retinal Explant
04:49Conclusion

Summary

Traduzione automatica

该协议描述了小鼠神经视网膜的细胞图外植，与视网膜色素上皮（RPE）一起在R16定义的介质中培养，不含血清和抗生素。与体内实验相比，这种方法性能相对简单、成本更低、耗时，可适应多种实验应用。

Tags

Organotypic Retina Explant Serum-free Culture Retinal Pigment Epithelium Retinitis Pigmentosa Diabetic Retinopathy In Vitro Retinal Model Pre-clinical Treatment Development

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