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使用人iPSC衍生的心肌细胞，心脏成纤维细胞和内皮细胞制造3D心脏微组织阵列
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JoVE Journal Bioingegneria
Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells
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使用人iPSC衍生的心肌细胞，心脏成纤维细胞和内皮细胞制造3D心脏微组织阵列

DOI:
10.3791/61879-v

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10:37 min

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March 14, 2021

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, , ,
1Stanford Cardiovascular Institute,Stanford University School of Medicine, 2Department of Medicine, Division of Cardiovascular Medicine,Stanford University School of Medicine, 3Department of Radiology,Stanford University School of Medicine

Capitoli

  • 00:04Introduction
  • 00:47Casting of the Cardiac Microtissue Molds and Cell Seeding
  • 03:32Fixation and Permeabilization of the Cells and the Cardiac Microtissues for Immunostaining
  • 05:45Digestion of the Cardiac Microtissues and Cell Preparation for the Flow Cytometry
  • 07:34Results: Estimation of the Purity, Morphology, the Contraction Profile of the Cells in the Cardiac Microtissues
  • 09:47Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里，我们描述了一种易于使用的方法，用于生成由预分化的人诱导多能干细胞衍生的心肌细胞，心脏成纤维细胞和内皮细胞组成的3D自组装心脏微组织阵列。这种用户友好且需要低细胞的技术来生成心脏微组织，可用于疾病建模和药物开发的早期阶段。

Tags

Human IPSC-derived CardiomyocytesCardiac FibroblastsEndothelial CellsAgarose MicromoldCardiac SpheroidsDisease ModelingDrug TestingContractile FunctionImagingCell SuspensionCell SeedingMedium Change

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