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Immunologia e infezioni

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用于剖析体外肿瘤微环境中免疫反应的微流控共培养模型

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments

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用于剖析体外肿瘤微环境中免疫反应的微流控共培养模型

DOI:

10.3791/61895-v

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07:46 min

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April 30, 2021

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Adele De Ninno, Francesca Romana Bertani, Annamaria Gerardino, Giovanna Schiavoni, Martina Musella, Claudia Galassi, Fabrizio Mattei, Antonella Sistigu⁴, Luca Businaro

¹CNR Institute for Photonics and Nanotechnology, ²Dept. of Oncology and Molecular Medicine,Istituto Superiore di Sanità, ³Istituto di Patologia Generale,Università Cattolica del Sacro Cuore, ⁴Tumor Immunology and Immunotherapy Unit,IRCCS Regina Elena National Cancer Institute

Capitoli

00:04Introduction
01:022D Chip Cell Plating
02:10Unlabeled Cancer and Immune Cell Time-Lapse Acquisition
02:433D Immuno-Competent Cancer On-Chip Model
05:15Results I: Migratory and Interaction Patterns of the 2D Tumor-Immune Cells Co-Culture by Time-Lapse Data Analysis
06:19Results II: Fluorescent Analysis of Infiltrated Immune Cells in a 3D Tumor Microenvironment
06:56Conclusion

Summary

Traduzione automatica

在免疫疗法和单细胞基因组分析时代，癌症生物学需要新颖的体外和计算工具，以便在适当的时空背景下研究肿瘤 - 免疫界面。我们描述了在2D和3D环境中利用肿瘤免疫微流体共培养的方案，与细胞功能的动态，多参数监测兼容。

Tags

Keywords: Microfluidic Co-culture Tumor Microenvironment Immune Response Cell Interactions 2D 3D Real-time Imaging Microscopy Tumor Cells Immune Cells Anti-cancer Treatments Matrigel Fluorescent Dyes

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