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Time-Resolved Fluorescence Imaging and Analysis of Cancer Cell Invasion in the 3D Spheroid Model
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Biologia
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JoVE Journal Biologia
Time-Resolved Fluorescence Imaging and Analysis of Cancer Cell Invasion in the 3D Spheroid Model

Time-Resolved Fluorescence Imaging and Analysis of Cancer Cell Invasion in the 3D Spheroid Model

DOI:
10.3791/61902-v

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07:42 min

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January 30, 2021

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, ,
1Bioengineering Department,Temple University, 2Cancer Biology Program,Fox Chase Cancer Center

Capitoli

  • 00:05Introduction
  • 00:39Spheroid Imaging Device (SID) Fabrication
  • 04:51Spheroid Collagen Embedding
  • 06:06Results: Representative Spheroid Imaging
  • 07:06Conclusion

Summary

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Presented here is a protocol for the fabrication of a spheroid imaging device. This device enables dynamic or longitudinal fluorescence imaging of cancer cell spheroids. The protocol also offers a simple image processing procedure for the analysis of cancer cell invasion.

Tags

Keywords: 3D Spheroid ModelCancer Cell InvasionTime-resolved Fluorescence ImagingExtracellular MatrixPDMS3D PrintingSpacerGlass PlatesVacuum ChamberBiopsy PunchMammary Carcinoma

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