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Xenopus laevis Egg Extract Preparation and Live Imaging Methods for Visualizing Dynamic Cytoplasmic Organization
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Biologia
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JoVE Journal Biologia
Xenopus laevis Egg Extract Preparation and Live Imaging Methods for Visualizing Dynamic Cytoplasmic Organization

Xenopus laevis Egg Extract Preparation and Live Imaging Methods for Visualizing Dynamic Cytoplasmic Organization

DOI:
10.3791/61923-v

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05:58 min

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June 06, 2021

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,
1Department of Chemical and Systems Biology,Stanford University School of Medicine, 2Current Address: Department of Biological Sciences,University of Southern California, 3Department of Biochemistry,Stanford University School of Medicine

Capitoli

  • 00:05Introduction
  • 00:35Preparation of Slides and Coverslips
  • 01:06Preparation and Live Imaging of Interphase-Arrested Egg Extracts
  • 04:06Result: Microscopy Analysis of Interphase-Arrested Egg Extracts Self-Organized into Cell-Like Compartments
  • 05:23Conclusion

Summary

Traduzione automatica

Traduzione automatica

We describe a method for the preparation and live imaging of undiluted cytoplasmic extracts from Xenopus laevis eggs.

Tags

Keywords: Xenopus LaevisEgg ExtractLive ImagingCytoplasmic OrganizationFEP TapeImaging SpacerDejellying SolutionMMR BufferEgg Lysis BufferCentrifugationAprotininLeupeptinCytochalasin BCyclohexamideCytoplasmic Layer

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