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Xenopus laevis Ægekstraktpræparation og levende billeddannelsesmetoder til visualisering af dynamisk cytoplasmisk organisation

JoVE Journal
Biologia

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JoVE Journal Biologia

Xenopus laevis Egg Extract Preparation and Live Imaging Methods for Visualizing Dynamic Cytoplasmic Organization

Xenopus laevis Ægekstraktpræparation og levende billeddannelsesmetoder til visualisering af dynamisk cytoplasmisk organisation

DOI:

10.3791/61923-v

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05:58 min

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June 06, 2021

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Xianrui Cheng², James E. Ferrell, Jr.³

¹Department of Chemical and Systems Biology,Stanford University School of Medicine, ²Current Address: Department of Biological Sciences,University of Southern California, ³Department of Biochemistry,Stanford University School of Medicine

Capitoli

00:05Introduction
00:35Preparation of Slides and Coverslips
01:06Preparation and Live Imaging of Interphase-Arrested Egg Extracts
04:06Result: Microscopy Analysis of Interphase-Arrested Egg Extracts Self-Organized into Cell-Like Compartments
05:23Conclusion

Summary

Traduzione automatica

Vi beskriver en metode til fremstilling og levende billeddannelse af ufortyndede cytoplasmatiske ekstrakter fra Xenopus laevis æg.

Tags

Keywords: Xenopus Laevis Egg Extract Live Imaging Cytoplasmic Organization FEP Tape Imaging Spacer Dejellying Solution MMR Buffer Egg Lysis Buffer Centrifugation Aprotinin Leupeptin Cytochalasin B Cyclohexamide Cytoplasmic Layer

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