Rivista
/
Genetica
/
Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator
JoVE Journal
Genetica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Genetica
Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator

Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator

DOI:
10.3791/61930-v

•

08:30 min

•

February 09, 2021

•

, , , , ,
1Department of Biology,University of Florida, 2Department of Biochemistry and Molecular Pharmacology,New York University School of Medicine, 3Department of Radiation Oncology,Columbia University Medical Center, 4Center for Smell and Taste,University of Florida

Capitoli

  • 00:05Introduction
  • 00:40Regular Maintenance of Harpegnathos saltator Colonies
  • 02:05Preparation of Quartz Glass Microinjection Needles and Microinjector
  • 04:10Injection of Embryos
  • 06:32Results: Mutagenesis Induction in H. saltator Ants
  • 07:37Conclusion

Summary

Traduzione automatica

Traduzione automatica

Many characteristics of insect eusociality rely on within-colony communication and division of labor. Genetic manipulation of key regulatory genes in ant embryos via microinjection and CRISPR-mediated mutagenesis provides insights into the nature of altruistic behavior in eusocial insects.

Tags

Keywords: CRISPRMutagenesisHarpegnathos SaltatorAntGenetic ModificationEmbryo InjectionMicroinjectionPlaster Nest BoxEusocial InsectsComportamentoCommunicationPhenotype PlasticityGraduate StudentAnt RearingColony MaintenanceWaste DisposalGlass Microinjection NeedlesCasein ProteinsSmall Guide RNAs

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/61930?article_type=v

Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata
check_url/it/61930?article_type=v

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
check_url/it/61930?article_type=v

Detection of Copy Number Alterations Using Single Cell Sequencing
check_url/it/61930?article_type=v

Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast
check_url/it/61930?article_type=v

Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation
check_url/it/61930?article_type=v

CRISPR-Mediated Reorganization of Chromatin Loop Structure
check_url/it/61930?article_type=v

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
check_url/it/61930?article_type=v

A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates
check_url/it/61930?article_type=v

Induction and Evaluation of Inbreeding Crosses Using the Ant, Vollenhovia Emeryi
check_url/it/61930?article_type=v

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans

Read Article