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使用基于曲线的转换工具量化纤维胶原蛋白组织

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Bioingegneria

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JoVE Journal Bioingegneria

Quantifying Fibrillar Collagen Organization with Curvelet Transform-Based Tools

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使用基于曲线的转换工具量化纤维胶原蛋白组织

DOI:

10.3791/61931-v

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07:58 min

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November 11, 2020

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Yuming Liu, Kevin W. Eliceiri^2,3

¹Laboratory for Optical and Computational Instrumentation, Center for Quantitative Cell Imaging,University of Wisconsin-Madison, ²Departments of Medical Physics and Biomedical Engineering,University of Wisconsin-Madison, ³Morgridge Institute for Research, Madison, Wisconsin, ⁴test,test

Capitoli

00:04Introduction
00:43CT-FIRE Fiber Extraction and Region of Interest (ROI) Analysis
02:34Advanced CT-FIRE Post-Processing
04:09CurveAlign Fiber Analysis: CT Mode Without Boundary
05:03CurveAlign Fiber Analysis: CT-FIRE Mode with Boundary
06:14CurveAlign Region of Interest (ROI) Analysis
06:56Results: Representative Tumor-Associated Human Breast Cancer Tissue Microarray (TMA) Collagen Signatures
07:26Conclusion

Summary

Traduzione automatica

在这里，我们提出了一个协议，使用基于曲线转换的开源 MATLAB 软件工具，用于量化正常组织和患病组织的细胞外基质中的纤维状胶原蛋白组织。此工具可应用于胶原纤维或其他类型的线状结构的图像。

Tags

Fibrillar Collagen Collagen Organization Extracellular Matrix Curvelet Transform CT-FIRE Image Analysis Fiber Extraction Region Of Interest Post-processing

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