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交替浸入葡萄糖中，在斑马鱼中产生长期血糖超血症

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Alternate Immersion in Glucose to Produce Prolonged Hyperglycemia in Zebrafish

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交替浸入葡萄糖中，在斑马鱼中产生长期血糖超血症

DOI:

10.3791/61935-v

•

05:49 min

•

May 05, 2021

•

Elizabeth McCarthy, Cassie J. Rowe², Mikayla Crowley-Perry³, Victoria P. Connaughton

¹Department of Biology,American University, ²Center for Behavioral Neuroscience,American University, ³Department of Chemistry,American University

Capitoli

00:05Introduction
00:57Preparing the Solution Tanks
02:07Transferring Fish
03:04Post-transfer Solution Preparation
03:53Results: Changes in Blood Glucose, GFAP Levels, and ERG Response in Zebrafish after Induction of Hyperglycemia
05:05Conclusion

Summary

Traduzione automatica

此协议非侵入性地诱发斑马鱼高血糖长达 8 周。利用这一协议，可以深入研究高血糖的不利影响。

Tags

Hyperglycemia Zebrafish Non-invasive Glucose Type 2 Diabetes Therapeutic Protocol Water Bath Housing Tank Solution Tank Water-to-water Mannitol Air Stone Tank Cleaning

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