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Funktionaliseret spirosyklisk heterocyklussyntese og cytotoksicitetsanalyse
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Biologia
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JoVE Journal Biologia
Functionalized Spirocyclic Heterocycle Synthesis and Cytotoxicity Assay

Funktionaliseret spirosyklisk heterocyklussyntese og cytotoksicitetsanalyse

DOI:
10.3791/61950-v

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05:17 min

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February 09, 2021

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, , , , ,
1Department of Biology and Chemistry,Azusa Pacific University

Capitoli

  • 00:04Introduction
  • 00:36Cytotoxicity Assay Using MTT
  • 03:39Results: Effects of Test Compounds on COS-7 Cell Viability in a Modified MTT Assay
  • 04:50Conclusion

Summary

Traduzione automatica

Traduzione automatica

Her beskriver vi et bioassay ved hjælp af 3-(4′,5′-dimethylthiazol-2′-yl)-2,5- diphenyltetrazoliumbromid (MTT) for at teste tidligere syntetiserede spirocykliske oximer.

Tags

Spirocyclic CompoundsMTT AssayCytotoxicitySolid-phase SynthesisHeterocyclesCell ViabilityDMSOFormazan CrystalsAbsorbanceCOS7 CellsCisplatin

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