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通过质谱学研究蛋白质高阶结构，与二乙基碳酸酯共价标签

JoVE Journal
Chimica
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JoVE Journal Chimica

Covalent Labeling with Diethylpyrocarbonate for Studying Protein Higher-Order Structure by Mass Spectrometry

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通过质谱学研究蛋白质高阶结构，与二乙基碳酸酯共价标签

DOI:

10.3791/61983-v

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10:36 min

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June 15, 2021

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Zachary J. Kirsch, Blaise G. Arden, Richard W. Vachet*^1,2, Patanachai Limpikirati*³

¹Department of Chemistry,University of Massachusetts Amherst, ²Molecular and Cellular Biology Program,University of Massachusetts Amherst, ³Department of Food and Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences,Chulalongkorn University

Summary

Traduzione automatica

介绍了在质谱检测中进行基于二乙基碳酸酯的共价贴标的实验程序。二甲基碳酸酯只是与感兴趣的蛋白质或蛋白质复合物混合，导致溶剂可获取氨基酸残留物的修饰。经蛋白解消化和液相色谱/质谱分析后，可识别修改后的残留物。

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Keywords: Covalent Labeling Diethylpyrocarbonate DEPC Mass Spectrometry Protein Structure Structural Characterization Buffer Exchange Imidazole

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