00:53Preparation of NE Depleted of U1 snRNP (U1ΔNE)
02:10Immunoprecipitation of 10S Fractions of Glycerol Gradients by Anti-Gal3
03:52Assembly of Splicing Reaction and Analysis of Products
06:09Results: RNA and Polypeptide Compositions of the Anti-Gal3 Precipitate of the 10S Region of the Glycerol Gradient and its Ability to Reconstitute Splicing in U1ΔNE
Este artigo descreve os procedimentos experimentais para (a) esgotamento do U1 snRNP a partir de extratos nucleares, com perda concomitante de atividade de emenda; e (b) reconstituição da atividade de emenda no extrato empobrecido U1 por partículas de galectina-3 - U1 snRNP ligadas a contas covalentemente acopladas a anticorpos anti-galectina-3.