È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:04Introduction
- 00:26Cloning Parts into the Entry Vector (pYTK001) to Create Part
- 02:05Assembling Part Plasmids into Cassette Plasmids
- 04:50Assembling Cassette Plasmids into Multi-Gene Plasmids
- 05:55Results: Multi-gene Plasmid Cloning in E. coli and Yeast
- 07:54Conclusion
The goal of this protocol is to provide a detailed, step-by-step guide for assembling multi-gene constructs using the modular cloning system based on Golden Gate cloning. It also gives recommendations on critical steps to ensure optimal assembly based on our experiences.
