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使用模块化金门克隆的多基因结构快速组装

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Bioingegneria

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JoVE Journal Bioingegneria

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

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使用模块化金门克隆的多基因结构快速组装

DOI:

10.3791/61993-v

•

08:31 min

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February 05, 2021

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Minakshi Mukherjee*¹, Emily Caroll*¹, Zhen Q. Wang

¹Department of Biological Sciences, University at Buffalo,State University of New York

Capitoli

00:04Introduction
00:26Cloning Parts into the Entry Vector (pYTK001) to Create Part
02:05Assembling Part Plasmids into Cassette Plasmids
04:50Assembling Cassette Plasmids into Multi-Gene Plasmids
05:55Results: Multi-gene Plasmid Cloning in E. coli and Yeast
07:54Conclusion

Summary

Traduzione automatica

该协议的目标是提供一个详细的，分步指南，以组装多基因结构使用模块化克隆系统的基础上，金门克隆。它还根据我们的经验就确保最佳装配的关键步骤提出了建议。

Tags

Multi-gene Constructs Modular Golden Gate Cloning Yeast Moclo Kit Cloning Protocol Golden Gate Reaction Pcr Products Entry Vector Dh5 Alpha E. Coli Chloramphenicol Sfgfp Intermediate Vector Mrfp1 Yeast Selection Marker Yeast Origin Of Replication Ampicillin Resistance

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