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Gonadectomy and Blood Sampling Procedures in the Small Size Teleost Model Japanese Medaka (Oryzias latipes)
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JoVE Journal Biologia
Gonadectomy and Blood Sampling Procedures in the Small Size Teleost Model Japanese Medaka (Oryzias latipes)

Gonadectomy and Blood Sampling Procedures in the Small Size Teleost Model Japanese Medaka (Oryzias latipes)

DOI:
10.3791/62006-v

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05:31 min

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December 11, 2020

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, , , , , ,
1Physiology Unit, Faculty of Veterinary Medicine,Norwegian University of Life Sciences, 2Laboratory of Physiology, Atmosphere and Ocean Research Institute,The University of Tokyo, 3Department of Biological Sciences, Graduate School of Science,The University of Tokyo, 4Centre of Reproduction, Development and Aging (CRDA), Faculty of Health Sciences,University of Macau

Capitoli

  • 00:04Introduction
  • 00:50Gonadectomy
  • 02:22Blood Sampling
  • 03:16Results: E2 and 11-KT Levels in Females and Males of Sham-Operated and Gonadectomized Fish
  • 04:54Conclusion

Summary

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The article describes a quick protocol to gonadectomize and sample blood from the small teleost fish, using Japanese medaka (Oryzias latipes) as a model, to investigate the role of sex steroids in animal physiology.

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Keywords: GonadectomyBlood SamplingJapanese MedakaTeleostOvariectomyOrchidectomyAnesthesiaIncisionSuturingBlood DrawCaudal Peduncle Vein

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