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Gonadectomy og blodprøvetaking prosedyrer i liten størrelse Teleost Modell Japansk Medaka (Oryzias latipes)
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JoVE Journal Biologia
Gonadectomy and Blood Sampling Procedures in the Small Size Teleost Model Japanese Medaka (Oryzias latipes)

Gonadectomy og blodprøvetaking prosedyrer i liten størrelse Teleost Modell Japansk Medaka (Oryzias latipes)

DOI:
10.3791/62006-v

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05:31 min

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December 11, 2020

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, , , , , ,
1Physiology Unit, Faculty of Veterinary Medicine,Norwegian University of Life Sciences, 2Laboratory of Physiology, Atmosphere and Ocean Research Institute,The University of Tokyo, 3Department of Biological Sciences, Graduate School of Science,The University of Tokyo, 4Centre of Reproduction, Development and Aging (CRDA), Faculty of Health Sciences,University of Macau

Capitoli

  • 00:04Introduction
  • 00:50Gonadectomy
  • 02:22Blood Sampling
  • 03:16Results: E2 and 11-KT Levels in Females and Males of Sham-Operated and Gonadectomized Fish
  • 04:54Conclusion

Summary

Traduzione automatica

Traduzione automatica

Artikkelen beskriver en rask protokoll for å gonadectomize og prøve blod fra den lille teleostfisken, ved hjelp av japansk medaka (Oryzias latipes) som modell, for å undersøke rollen som sexsteroider i dyrefysiologi.

Tags

Keywords: GonadectomyBlood SamplingJapanese MedakaTeleostOvariectomyOrchidectomyAnesthesiaIncisionSuturingBlood DrawCaudal Peduncle Vein

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