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Fluorescence In Situ Hybridization on DNA Halo Preparations to Reveal Whole Chromosomes, Telomeres and Gene Loci
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Fluorescence In Situ Hybridization on DNA Halo Preparations to Reveal Whole Chromosomes, Telomeres and Gene Loci
DOI:

09:07 min

March 04, 2021

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Capitoli

  • 00:04Introduction
  • 00:46Slide Preparation and Cell Culture
  • 02:08DNA Halo Preparation
  • 03:22Two‐Dimensional Fluorescence In Situ Hybridization
  • 06:47Results: DNA Halos for High‐Resolution Analysis of Genomic Interactions with the Nucleoskeleton
  • 08:41Conclusion

Summary

Traduzione automatica

Combining DNA halo preparations with fluorescence in situ hybridization enables high-resolution analysis of genomic interactions with the nucleoskeleton. Attached genome leads to hybridized fluorescent signals located within the residual extracted nuclei, whereas non-attached genome is in the halo of DNA surrounding the residual nuclei.

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