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1Laboratory of Nuclear and Genomic Health, Centre for Genome Engineering and Maintenance, Division of Biosciences, Department of Life Sciences, College of Health, Medicine and Life Sciences,Brunel University London, 2Biosciences, Department of Clinical, Pharmaceutical and Biological Science, School of Life and Medical Sciences,University of Hertfordshire
Capitoli
- 00:04Introduction
- 00:46Slide Preparation and Cell Culture
- 02:08DNA Halo Preparation
- 03:22Two‐Dimensional Fluorescence In Situ Hybridization
- 06:47Results: DNA Halos for High‐Resolution Analysis of Genomic Interactions with the Nucleoskeleton
- 08:41Conclusion
Genom att kombinera DNA-halopreparat med fluorescens in situ-hybridisering möjliggörs högupplöst analys av genomiska interaktioner med nukleoskelettet. Bifogat genom leder till hybridiserade fluorescerande signaler belägna inom de kvarvarande extraherade kärnorna, medan icke-bundet genom ligger i DNA-halo som omger restkärnorna.
