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A Murine Ommaya Xenograft Model to Study Direct-Targeted Therapy of Leptomeningeal Disease
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JoVE Journal Ricerca sul cancro
A Murine Ommaya Xenograft Model to Study Direct-Targeted Therapy of Leptomeningeal Disease

A Murine Ommaya Xenograft Model to Study Direct-Targeted Therapy of Leptomeningeal Disease

DOI:
10.3791/62033-v

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07:17 min

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January 29, 2021

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, , , , , , , , , ,
1Department of Neuro-Oncology,H. Lee Moffitt Cancer Center & Research Institute, 2Department of Tumor Biology,H. Lee Moffitt Cancer Center & Research Institute, 3Department of Comparative Medicine,University of South Florida, 4Department of Breast Oncology,H. Lee Moffitt Cancer Center & Research Institute, 5Department of Cancer Physiology,H. Lee Moffitt Cancer Center & Research Institute, 6Department of Drug Discovery,H. Lee Moffitt Cancer Center & Research Institute, 7Department of Medical Oncology,Roswell Park Comprehensive Cancer Center

Capitoli

  • 00:04Introduction
  • 01:07Injection of CTCs into CSF to Generate a Mouse LMD Model
  • 03:37Murine Ommaya Assembly and Implantation
  • 05:20Results: Direct-Targeted Immunotherapy Using the Murine Ommaya Increases the Overall Survival of Breast Cancer-Associated Leptomeningeal Disease Mice
  • 06:37Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we describe a murine xenograft model that functionally resembles an Ommaya reservoir in patients. We developed the Murine Ommaya to study novel therapeutics for the universally fatal leptomeningeal disease.

Tags

Keywords: Murine OmmayaXenograft ModelLeptomeningeal DiseaseDirect-targeted TherapyBrain MetastasesCerebrospinal FluidBlood-brain BarrierStereotactic SurgeryBuprenorphineCisterna MagnaHamilton SyringeDural Membrane

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