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Assembly and Operation of an Acoustofluidic Device for Enhanced Delivery of Molecular Compounds to Cells
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Bioingegneria
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JoVE Journal Bioingegneria
Assembly and Operation of an Acoustofluidic Device for Enhanced Delivery of Molecular Compounds to Cells

Assembly and Operation of an Acoustofluidic Device for Enhanced Delivery of Molecular Compounds to Cells

DOI:
10.3791/62035-v

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07:16 min

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January 21, 2021

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, , , , , , , ,
1Department of Bioengineering,University of Louisville, 2School of Medicine,University of Louisville, 3Department of Biology,University of Louisville

Capitoli

  • 00:04Introduction
  • 00:45Fabrication of Acoustofluidic Device
  • 02:18Assembly and Operation of Acoustofluidic System
  • 04:08Preparation of Ultrasound Contrast Agents
  • 05:38Results: Acoustofluidic Treatment Enhances Intracellular Molecular Delivery
  • 06:30Conclusion

Summary

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This protocol describes the assembly and operation of a low-cost acoustofluidic device for rapid molecular delivery to cells via sonoporation induced by ultrasound contrast agents.

Tags

Acoustofluidic DeviceBiomolecule DeliveryCell TransformationPDMSPhotoresist WaferPiezo TransducerMicrocontroller8 MHz SignalTygon Tubing

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