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使用硫二甘酸(TGA)估计植物生物质木质素含量
JoVE Journal
Biochimica
Author Produced
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JoVE Journal Biochimica
Estimation of Plant Biomass Lignin Content using Thioglycolic Acid (TGA)
DOI:

09:25 min

July 24, 2021

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Capitoli

  • 00:00Introduction
  • 01:15Protocol: Phase I- Preparation of Plant Samples
  • 02:34Protocol: Phase II- Preparation of Alcohol Insoluble Material (Water and Methanol Washes)
  • 03:46Protocol: Phase III- Treatment of Pellet with TGA and Acid to Precipitate Lignin
  • 06:22Protocol: Phase IV- Results (Ab280mm Reading)
  • 07:01Representative Results
  • 08:38Conclusion

Summary

Traduzione automatica

在这里,我们提出了一个修改后的TGA方法,用于估计草本植物生物质中的木质素含量。这种方法通过与木质素形成特定的硫化物键来估计木质素含量,并且比克拉森方法具有优势,因为它需要相对较小的木质素含量估计样本。

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